Thermo Scientific™

LanthaScreen™ TR-FRET TR alpha Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15136:The LanthaScreen™ TR-FRET TR (thyroid receptor)자세히 알아보기

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15136:

The LanthaScreen™ TR-FRET TR (thyroid receptor) alpha Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential TR alpha ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant TR alpha ligand-binding domain (TR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.

To Assay:

When performing the LanthaScreen™ TR-FRET TR alpha coactivator assay, TR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the TR alpha-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, induce a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:

The LanthaScreen™ TR-FRET TR alpha Coactivator Assay Kit contains TR alpha-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

사양

콘주게이트Tb (Terbium)

검출 방법Fluorescence

용도(장비)Microplate Reader

수량800 x 20 μL assays

포장384-well Plate

어세이 입력Biochemical coregulator interaction

판독End Point

배송 조건Dry Ice

타겟 입력THRA, TR alpha, NR1A1

제품라인LanthaScreen™

용도(애플리케이션)Co-Factor Interaction Assay, TR-FRET

리간드TR alpha

제품 유형TR-FRET TR Alpha Coactivator Assay Kit

No. of Assays800 x 20 μL

Unit SizeEach