Thermo Fisher Scientific RIP1 Polyclonal Antibody
RIP1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합. 고순도 항원 친화 크로마토그래피로 정제된 액상 제품으로, 1.0 mg/mL 농도 유지. Human, Mouse, Rat에 반응.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 7 publications |
| Immunohistochemistry (IHC) | 2.5 µg/mL | View 2 publications |
| Immunocytochemistry (ICC/IF) | 20 µg/mL | - |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 15 amino acid peptide from near the amino terminus of human RIPK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154790 |
Product Specific Information
- Suggested positive control: rat kidney tissue lysate
- PA5-20811 can be used with blocking peptide PEP-0925
Target Information
RIPK1 is a serine-threonine kinase that transduces inflammatory and cell-death signals following death receptor ligation, activation of pathogen recognition receptors (PRRs), and DNA damage.
Upon activation of TNFR1 by TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. It phosphorylates DAB2IP at Ser-728 in a TNF-alpha-dependent manner, activating the MAP3K5-JNK apoptotic cascade.
Ubiquitination by TRAF2 via Lys-63-linked chains enhances downstream signaling through MAPK and NF-kappa-B pathways, leading to expression of inflammatory molecules.
Polyubiquitinated RIPK1 binds to IKBKG/NEMO, a key step for NF-kappa-B activation. Interaction with RHIM-containing adapters triggers gene activation and cell death. RIPK1 and RIPK3 association forms a necrosis-inducing complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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