Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204) Recombinant Rabbit Monoclonal Antibody (ERK12T202Y204-A11)
ERK1/ERK2 인산화 상태(Thr202/Tyr204)를 특이적으로 인식하는 Thermo Fisher Scientific의 재조합 토끼 단일클론 항체. Western blot 및 Flow cytometry에 적합하며, HEK293 세포에서 발현된 IgG 형식. 단백질 인산화 신호전달 연구에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Flow Cytometry (Flow) | 1–1,000 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ERK12T202Y204-A11 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr202/Tyr204 of human phospho Erk1/2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 50% glycerol, 0.10% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897148 |
Additional Formats
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for G protein-coupled receptors, and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to receptor tyrosine kinases, Ras interacts with Raf, activating it as a serine/threonine protein kinase. Activated Raf phosphorylates MEK1/2, which in turn phosphorylates ERK1/2. Upon activation, ERKs phosphorylate cytoplasmic targets or migrate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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