Thermo Fisher Scientific H4K16ac Monoclonal Antibody (GT1271), ChIP-Verified
H4K16ac 단일클론 항체(GT1271)는 ChIP 검증 완료된 항체로, Western blot, IHC, ICC/IF, ChIP assay에 적합합니다. 인간 및 생쥐 시료에 반응하며, Protein A로 정제된 1 mg/mL 농도의 액상 제품입니다. 히스톤 H4의 N-말단 아세틸화 리신16 인식.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| ChIP assay (ChIP) | 1–5 µg | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT1271 |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4 (acetyl Lys16). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2866506 |
Product Specific Information
- Positive Control: HeLa (untreated), HeLa (0.4 µM Trichostatin A for 18 hr)
- Predicted Reactivity: Xenopus laevis (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Master Lot Certificate (PDF)
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in chromatin compaction. This gene is intronless and encodes a member of the histone H4 family. Transcripts lack polyA tails but contain a palindromic termination element. The gene is located in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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