Thermo Fisher Scientific IL1F6 Monoclonal Antibody (OTI3A2), TrueMAB
인간 IL1F6 단백질을 타겟으로 하는 Mouse IgG1 단일클론 항체로, WB, ICC/IF, Flow Cytometry에 사용 가능. 동결건조 형태로 제공되며, 1 mg/mL 농도. 연구용으로 NF-κB 및 MAPK 경로 관련 염증 반응 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A2 |
| Immunogen | Full length human recombinant protein of IL1F6 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The IL-1 family consists of 11 structurally related ligands, including IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9).
IL-36α is highly expressed in psoriatic plaques and uniquely expressed by T cells, monocytes, and B cells. It is primarily found in skin and lymphoid tissues, and to a lesser extent in fetal brain, trachea, stomach, and intestine.
IL-36α signals through IL-1Rrp2 (IL-1R6) and IL-RAcP receptors, functioning as an agonist of NF-κB and MAPK pathways, inducing proinflammatory cytokines and chemokines such as IL-6, IL-8, BD-2, and BD-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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