Thermo Fisher Scientific AHR Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) [IHC (P)]

• Tested Dilution: 6 µg/mL

Product Specifications

Product Specific Information

• Percent identity with other species by BLAST analysis:
  Human, Gorilla (100%); Gibbon, Monkey, Horse (94%); Marmoset, Bovine (88%); Bat, Dog, Hamster, Panda, Guinea pig (81%)
• For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer.
• After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

AHR (Ah Receptor) belongs to a family of proteins that includes its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM.
AHR contains an N-terminal PAS domain (~200 amino acids) and binds specific DNA enhancer sequences to initiate transcription of cytochrome P-450 (CYPIA1) mRNA.
It acts as a ligand-activated transcription factor regulating biological responses to planar aromatic hydrocarbons.

Known ligands include polycyclic and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).
In non-ligand activated cells, AHR is complexed with HSP90 in the cytoplasm. Upon ligand binding, AHR transforms into a nuclear DNA-binding form, dissociates from HSP90, and forms a heterodimer with Arnt.

Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.