Thermo Fisher Scientific HIF1 alpha Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

• Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
• The antigen shares 94% identity with rat and mouse sequences.
• Specificity: HIF1A.

Target Information

HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under reduced oxygen tension.
HIF-1 is a heterodimer consisting of alpha and beta subunits, both belonging to the PAS family of transcription factors.
HIF1 regulates the adaptive response to hypoxia by activating transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, and vascular endothelial growth factor (VEGF).
It plays roles in hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis.
Under hypoxic conditions, HIF1-alpha interacts with p53 and undergoes MDM2-mediated ubiquitination and degradation.
Induction of NOX4 by HIF1-alpha helps maintain ROS levels post-hypoxia and supports hypoxia-induced proliferation.
In humans, HIF1A is located on chromosome 14q.
Its C-terminal binds to p300, forming complexes that induce hypoxia-responsive genes such as VEGF.
Hypoxia contributes to diseases including ischemia, cancer, pulmonary hypertension, congenital heart disease, and COPD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.