Thermo Fisher Scientific Cullin 3 Polyclonal Antibody
Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능한 Rabbit Polyclonal Anti-Cullin 3 항체. Human, Mouse, Rat 반응성. Protein A로 정제된 액상 항체로, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human Cullin 3 (amino acids 411–510) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cullin 3 is a member of the family of human cullin genes (CUL1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc53 gene.
CUL1 forms a complex with human p19 skp1 and F box protein p45 skp2. Together with cdc53 (known as Cul A), these three subunits form a ubiquitin ligase that controls proteolysis of G1 cell cycle regulatory proteins.
In contrast, CUL2 does not associate with p19 skp1 and p45 skp2 but forms a complex with the inactive transcriptional elongation complex SIII, composed of Elongin C, Elongin B, and VHL. The function of this association remains unknown.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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