Thermo Fisher Scientific 4EBP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-118 of human eIF4EBP1 (NP_0040861) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:4EBP1,uniProtId:Q13541-1,ncbiNodeId:9606,antigenRange:50-118,antigenLength:118,antigenImageFileName:PA5-120165_4EBP1_Q13541-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120165_4EBP1_Q13541-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.14 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913737

Product Specific Information

Positive test controls include: MCF7, HeLa, SH-SY5Y, NIH/3T3.

Immunogen sequence: TRIIYDRKFL MECRNSPVTK TPPRDLPTIP GVTSPSSDEP PMEASQSHLR NSPEDKRAGG EESQFEMDI

Target Information

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E, which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5` end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.