Thermo Fisher Scientific MDM2 Monoclonal Antibody (OTI4E5), TrueMAB

Applications

• Western Blot (WB)
  Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

MDM2 is a ubiquitin ligase for p53, playing a central role in regulating p53 stability. It is located on chromosome 12q.
Phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, promoting p53 ubiquitination and degradation.
MDM2 also blocks binding to p19ARF, further enhancing p53 degradation. Overexpression of MDM2 can inactivate p53 and reduce its tumor suppressor function.
In addition to p53, MDM2 interacts with proteins such as retinoblastoma 1 and ribosomal protein L5, influencing cell cycle, apoptosis, and tumorigenesis.
More than 40 alternatively spliced transcript variants of MDM2 have been identified in both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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