Thermo Fisher Scientific Elk1 Polyclonal Antibody
ELK1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, IP 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. Affinity chromatography로 정제된 액상 제품이며, PBS/glycerol buffer에 보관. 연구용 전용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ELK1 (Accession P19419), corresponding to amino acid residues T363–D413 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818447 |
Product Specific Information
This antibody detects endogenous levels of total ELK1.
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. It is phosphorylated by MAP kinase pathways at multiple S/T motifs near the C-terminus, with phosphorylation at Ser383 being critical for transcriptional activation. ELK1 serves as a nuclear target for the Ras-Raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been identified.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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