Thermo Fisher Scientific SETD2 Monoclonal Antibody (OTI1C4), TrueMAB

Thermo Fisher Scientific SETD2 Monoclonal Antibody (OTI1C4), TrueMAB

Applications

• Western Blot (WB)
  Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

• For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
• For conjugation experiments, perform desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882) before use.

Target Information

SETD2 is a histone methyltransferase that specifically trimethylates Lys-36 of histone H3 (H3K36me3) using dimethylated Lys-36 (H3K36me2) as substrate. It is the main enzyme generating H3K36me3, a marker for epigenetic transcriptional activation.
SETD2 plays roles in:

• Chromatin structure modulation during transcriptional elongation
• DNA mismatch repair in G1 and early S phase via recruitment of the MutS alpha complex
• Maintenance of heterochromatin through DNMT3A recruitment
• Coupling of splicing and transcription elongation
• Angiogenesis regulation
• Recruitment to adenovirus 12 E1A gene promoters during infection

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.