Thermo Fisher Scientific NR3C2 Monoclonal Antibody (H10E4C9F)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA1-620 detects mineralocorticoid receptor (MR) in human, rat, rabbit, and chicken tissues.
It has been successfully used in Western blot, immunocytochemistry, and immunohistochemistry applications.
By Western blot, this antibody detects a 116 kDa protein representing MR in rat heart homogenate.
Immunohistochemical staining of MR in rabbit atrium with MA1-620 results in strong staining of myocytes and endothelial cells.
MA1-620 cannot be used for immunoprecipitation.

In immunohistochemical studies, staining with MA1-620 is blocked by pre-incubating the sample with aldosterone.
It can also be used in formalin-fixed, paraffin-embedded sections.
Due to the low levels of MR expressed in native tissues, enhanced detection systems (e.g., enhanced chemiluminescence) are recommended for Western blotting.
MA1-620 blocks aldosterone binding since it targets the steroid binding domain of the mineralocorticoid receptor.
The antibody may also react with the steroid binding domain for aldosterone on other receptors.
The immunogen used is aldosterone-3, produced using the anti-idiotypic method.

Target Information

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to DNA sequences following activation by their respective hormones.
Mineralocorticoids, secreted by the adrenal cortex, regulate metabolic processes including electrolyte balance.
They act through the mineralocorticoid receptor (MR), which associates with DNA to exert its effects.
The kidney is a primary target organ for mineralocorticoids, containing abundant MR.
The corresponding gene for the mineralocorticoid receptor is NR3C2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.