Thermo Fisher Scientific WEE1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 274 and 506 of Human WEE1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:WEE1,uniProtId:P30291-1,ncbiNodeId:9606,antigenRange:274-506,antigenLength:646,antigenImageFileName:PA5-29303_WEE1_P30291-1_Rabbit.svg,antigenImageFileNamePDP:PA5-29303_WEE1_P30291-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.83 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2546779

Product Specific Information

Recommended positive controls: Jurkat, Raji, NCI-H929, Neuro2A, C8D30, C2C12.

Predicted reactivity: Mouse (95%), Rat (96%), Xenopus laevis (84%), Chicken (88%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

WEE1, which is classified as a Ser/Thr kinase by sequence analysis, has been shown to inactivate multiple CDKs by tyrosine phosphorylation. Wee1 protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.