Thermo Fisher Scientific GCN1L1 Polyclonal Antibody

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
ALKEKLGTPD EQLEMANCQA VILSVEDDTG HRIIIEDLLE ATRSPEVGMR QAAAIILNIY CSRSKADYTS HLRSLVSGLI RLFNDSSPVV LEESWDALNA ITKKLDAGNQ LALIEELHKE IRLIGNESKG EHVPGFC

Highest antigen sequence identity to the following orthologs:

• Mouse: 97%
• Rat: 96%

Target Information

Acts as a positive activator of the EIF2AK4/GCN2 protein kinase activity in response to amino acid starvation. Forms a complex with EIF2AK4/GCN2 on translating ribosomes; during this process, GCN1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of EIF2AK4/GCN2, and hence stimulating EIF2AK4/GCN2 kinase activity. Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator ATF4, by promoting the EIF2AK4/GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on Ser-52, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. [UniProt]

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.