Thermo Fisher Scientific BTRC Monoclonal Antibody (OTI1F10), TrueMAB

Thermo Fisher Scientific BTRC Monoclonal Antibody (OTI1F10), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

The BTRC gene encodes a member of the F-box protein family, characterized by a 40-amino-acid F-box motif.
F-box proteins are part of the SCF (SKP1–Cullin–F-box) ubiquitin ligase complex involved in phosphorylation-dependent ubiquitination.
They are classified into three groups:

• Fbws: containing WD-40 domains
• Fbls: containing leucine-rich repeats
• Fbxs: containing diverse protein-protein interaction modules or no recognizable motifs

The BTRC protein belongs to the Fbws class, containing multiple WD-40 repeats.
It is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2, and Drosophila Slimb proteins.
BTRC interacts with HIV-1 Vpu, linking CD4 to the proteolytic machinery, and associates with phosphorylated IκBα and β-catenin destruction motifs.
It functions in multiple transcriptional programs by activating the NF-κB pathway and inhibiting the β-catenin pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.