Thermo Fisher Scientific Beta-2-microglobulin (B2M) Human ProcartaPlex Simplex Kit

Invitrogen™ Beta-2-microglobulin (B2M) Human ProcartaPlex™ Simplex Kit

The Human Beta-2-microglobulin (B2M) Simplex ProcartaPlex Kit measures beta-2-microglobulin (B2M) protein and is designed to be combinable with other Simplex kits, allowing creation of custom multiplex panels utilizing Luminex xMAP technology for protein detection and quantitation.

When combining multiple Simplex kits (e.g., when not using a pre-configured multiplex panel), only one buffer kit (Cat. No. EPX010-10420-901) is required per assay plate regardless of plex size.

Recommended sample pre-dilution for serum and plasma samples: 1:100

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on sandwich ELISA principles, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously via a Luminex instrument.

ProcartaPlex multiplex assays require as little as:

• 25 μL of plasma or serum
• 50 μL of cell culture supernatant
  and yield analyzed results within 4 hours.

Key Features

• Reproducible, reliable results — validated to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample — measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology — the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures identifiable by Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX).

Similar to a sandwich ELISA, matched antibody pairs identify the protein of interest. Each spectrally unique bead is labeled with antibodies specific to a single target protein, and bound proteins are detected using biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). This enables analysis of multiple targets in a single well.

The key difference from ELISA is that the capture antibody in ProcartaPlex assays is conjugated to magnetic beads rather than adsorbed to microplate wells, allowing reagents to remain free-floating in solution. The Luminex instrument uses two lasers—one to distinguish bead spectral signatures and another to quantify RPE fluorescence proportional to protein amount.

ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time as a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available across six species:

• Human
• Mouse
• Rat
• Nonhuman primate
• Porcine
• Canine

For more information and available products, visit thermofisher.com/procartaplex.

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