
Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488
DyLight 488 형광 표지를 가진 염소 유래 항-마우스 IgG(H+L) 2차 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 면역실험에 사용 가능. 높은 특이성과 감도를 제공하며, 492/519 nm의 형광 특성을 가짐. 연구용으로만 사용.
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Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| Flow Cytometry (Flow) | 1:25–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Mouse IgG, whole molecule |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C (protected from light) |
| Shipping Conditions | Ambient |
| RRID | AB_844397 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #35502 has been successfully used in Western blot, IF, ICC, IHC, IP, and FACS applications.
This antibody reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. Cross-reactivity with immunoglobulins from other species may occur.
Store protected from light at 4°C until opened. To extend shelf life, add an equal volume of glycerol to reach ~50% final concentration and store at -20°C.
DyLight 488 Amax = 493 nm; Emax = 518 nm. Mole Dye/Mole Protein Ratio is lot-dependent.
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies enable multiple detection systems (e.g., HRP, AP, fluorescence) and offer enhanced sensitivity through signal amplification.
These antibodies are typically generated by immunizing host animals with pooled immunoglobulins from the target species and purified via immunoaffinity chromatography or conjugation to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats Available
- Agarose
- Alexa Fluor 488, 555, 647, 680, 790
- Alexa Fluor Plus 405, 488, 647
- DyLight 350, 405, 550, 594, 633, 650, 680, 800 4X PEG
- FITC, HRP, PE, TRITC, Rhodamine, bGal, Biotin, AP
- WesternDot 625
- Custom conjugation available upon request
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