Thermo Fisher Scientific Goat anti-Rabbit IgG Fab Recombinant Secondary Antibody
Recombinant goat anti-rabbit IgG Fab secondary antibody. High specificity with no cross-reactivity to human, rat, or mouse IgG. Suitable for WB and ELISA. Provides consistent performance and animal origin-free formulation. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| ELISA | 0.01–0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | RMG01 |
| Immunogen | Peptide corresponding to Rabbit IgG |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.2–7.4, with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2665367 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody reacts to the Fab region of Rabbit IgG and shows no cross-reactivity with human, rat, or mouse IgG.
Recombinant antibodies are produced using specific genes encoding the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. Advantages of recombinant antibodies include better specificity, animal origin-free formulation, and consistent performance between lots.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified reagents with high specificity for rabbit immunoglobulins. They are used for detection, sorting, or purification of rabbit IgG targets. Secondary antibodies enhance versatility and sensitivity by enabling various detection systems (e.g., HRP, AP, fluorescence) and amplifying signals as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host with pooled rabbit immunoglobulins and may be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for specific applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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