Thermo Fisher Scientific AMPK alpha 2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (IHC)

1:50-1:250

-

ELISA (ELISA)

1:10,000

-

Immunoprecipitation (IP)

1:50-1:250

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide taken within amino acid region 480-530 on rat 5-AMP-activated protein kinase catalytic subunit alpha-2 protein. if (typeof window.$mangular ===undefined|| !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson =[{targetFamily:AMPK alpha-2,uniProtId:Q09137-1,ncbiNodeId:10114,antigenRange:480-530,antigenLength:552,antigenImageFileName:AMPK2-201AP\_AMPK\_alpha-2\_Q09137-1\_Rabbit.svg,antigenImageFileNamePDP:AMPK2-201AP\_AMPK\_alpha-2\_Q09137-1\_Rabbit\_PDP.jpeg,sortOrder:2}]; $mangular.isB2BCMGT = ``false``; $mangular.isEpitopesModalImageMultiSizeEnabled =true`;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5-1.5 mg/mL

Purification

Affinity chromatography

Storage buffer

proprietary buffer, pH 7.4-7.8, with 0.5% BSA, 30% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.