Thermo Fisher Scientific CD80 Monoclonal Antibody (MEM-233), FITC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

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Miscellaneous PubMed (Misc)

-

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MEM-233

Immunogen

Human CD80

Conjugate

FITC FITC FITC

View additional formats

• PE
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10372360

Product Specific Information

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

Analyte Specific Reagent