Thermo Fisher Scientific gamma Crystallin Polyclonal Antibody
Rabbit polyclonal antibody against human gamma C Crystallin (CRYG3); validated for WB, IHC, ICC, and ELISA; protein A purified, liquid form; ideal for lens protein research and cataract studies.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human gamma C Crystallin (CRYG3), amino acids 101–174 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Crystallins are divided into two classes: taxon-specific (enzyme) and ubiquitous. The ubiquitous class forms the major proteins of the vertebrate eye lens, maintaining transparency and refractive index. Lens fiber cells lose their nuclei during development, and crystallins are retained throughout life, making them extremely stable. Mammalian lens crystallins are grouped into alpha, beta, and gamma families; beta and gamma form a superfamily. Gamma-crystallins are monomeric, highly symmetrical proteins lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Gamma-crystallin genes (gamma-A through gamma-D) and pseudogenes (gamma-E, gamma-F, gamma-G) exist as a tandem gene cluster. Mutations or aging affecting gamma-crystallins are associated with cataract formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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