Thermo Fisher Scientific Endothelium Monoclonal Antibody (EN4)

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

1:20

-

Flow Cytometry (Flow)

1:20

-

Product Specifications

Species Reactivity

Cat, Guinea pig, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

EN4

Immunogen

Proprietary Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

purified

Storage buffer

PBS with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073014

Product Specific Information

This antibody stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen which is lost. It stains strongly murine fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells it recognizes the 130 kD CD31 antigen. EN4 also binds to guinea-pig and cat endothelium, but not to rabbit, bovine, sheep, dog, rat or mice endothelial cells. No other structures in the skin, heart, kidney, tonsils or spleen are stained with this antibody.

Target Information

The endothelium is the layer of thin specialized epithelium, comprising a simple squamous layer of cells that line the interior surface of blood vessels, forming an interface between circulating blood in the lumen and the rest of the vessel wall (simple squamous epithelium). Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. These cells reduce friction of the flow of blood.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.