Thermo Fisher Scientific HNMT Polyclonal Antibody
Human HNMT 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot 및 IHC(P) 검증 완료. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol buffer에 저장. 연구용으로만 사용 가능.
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Thermo Fisher Scientific HNMT Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human HNMT. Recombinant protein control fragment (Product #RP-102666). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2642491 |
Product Specific Information
Immunogen sequence:
TSDDLTQMLD NLGLKYECYD LLSTMDISDC FIDGNENGDL LWDFLTETCN FNATAPPDLR AELGKDLQEP EFSAKKEGKV LFNNTLSFIV IEA
Highest antigen sequence identity to orthologs:
- Mouse: 83%
- Rat: 83%
Target Information
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase (HNMT) and oxidative deamination via diamine oxidase. HNMT is a cytosolic enzyme using S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, histamine neurotransmitter activity is regulated by N(tau)-methylation since diamine oxidase is absent in the central nervous system. A common genetic polymorphism affects the activity levels of this enzyme in red blood cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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