Thermo Fisher Scientific SKA3 Polyclonal Antibody

Applications and Tested Dilution

• Application: Western Blot (WB)
• Tested Dilution: 0.2–1 µg/mL

Product Specifications

Product Specific Information

• Immunogen sequence: EVEDRTSLVL NSDTCFENLT DPSSPTISSY ENLLRTPTPP EVTKIPEDIL
• Storage guidance:
  • Short term: store at 2–8°C up to 1 week
  • Long term: store at -20°C in small aliquots to prevent freeze-thaw cycles
• Predicted homology:
  • Cow: 93%
  • Dog: 100%
  • Guinea Pig: 100%
  • Horse: 100%
  • Human: 100%
  • Rabbit: 100%
  • Rat: 86%

Target Information

Upon entry into mitosis, the cell’s microtubule network forms the mitotic spindle, enabling chromosome segregation. Kinetochores (KT) on centromeric chromatin are essential for proper attachment of chromosomes to spindle microtubules.
The spindle and kinetochore complex (SKA), composed of SKA1, SKA2, and SKA3, is required for stable KT-MT interactions and timely anaphase onset.
Depletion of SKA3 delays anaphase transition, leading to a prolonged metaphase-like state and accumulation of checkpoint protein Bub1 at kinetochores, indicating the SKA complex’s role in spindle checkpoint silencing and chromosome cohesion maintenance during mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.