Thermo Fisher Scientific HMGA1 Recombinant Rabbit Monoclonal Antibody (ARC1060)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000-1:8,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

ChIP assay (ChIP)

3 µg antibody for 10 µg-15 µg of Chromatin

-

ChIP-sequencing (ChIP-Seq)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC1060

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-107 of human HMGA1 (P17096) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HMGA1,uniProtId:P17096-1,ncbiNodeId:9606,antigenRange:1-107,antigenLength:107,antigenImageFileName:MA5-35485_HMGA1_P17096-1_Rabbit.svg,antigenImageFileNamePDP:MA5-35485_HMGA1_P17096-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.6 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849386

Product Specific Information

Immunogen sequence: MSESSSKSSQ PLASKQEKDG TEKRGRGRPR KQPPVSPGTA LVGSQKEPSE VPTPKRPRGR PKGSKNKGAA KTRKTTTTPG RKPRGRPKKL EKEEEEGISQ ESSEEEQ

Target Information

HMGA1 encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.