Thermo Fisher Scientific HGK Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 480-650 of human MAP4K4 (NP_6637192) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HGK,uniProtId:O95819-1,ncbiNodeId:9606,antigenRange:480-650,antigenLength:1239,antigenImageFileName:PA5-88221_HGK_O95819-1_Rabbit.svg,antigenImageFileNamePDP:PA5-88221_HGK_O95819-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4.69 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804754

Product Specific Information

Immunogen sequence: EVLQQQLLQE QAMLLHDHRR PHPQHSQQPP PPQQERSKPS FHAPEPKAHY EPADRAREVE DRFRKTNHSS PEAQSKQTGR VLEPPVPSRS ESFSNGNSES VHPALQRPAE PQVPVRTTSR SPVLSRRDSP LQGSGQQNSQ AGQRNSTSSI EPRLLWERVE KLVPRPGSGS S; Positive Samples: U-87MG, HeLa; Cellular Location: Cytoplasm

Target Information

MAP4K4 (HGK) is a serine/threonine kinase which plays an important role in stress-activated MAPK core signaling pathways, the response to environmental stress and cytokines such as TNF-alpha. It appears to act upstream of the JUN N-terminal pathway. This protein is thought to interact with the SH3 domain of the adapter proteins Nck. HGK binds, via its CNH regulatory domain, to the N-terminal region of SPG3A. Expression appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, and isoform 4 is predominant in the liver, skeletal muscle and placenta.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.