Thermo Fisher Scientific Rubicon Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

-

Immunoprecipitation (IP)

1:100-1:500

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 677 and 972 of Rubicon if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Rubicon,uniProtId:Q92622-1,ncbiNodeId:9606,antigenRange:677-972,antigenLength:972,antigenImageFileName:PA5-35969_Rubicon_Q92622-1_Rabbit.svg,antigenImageFileNamePDP:PA5-35969_Rubicon_Q92622-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.48 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2553279

Product Specific Information

Recommended positive controls: MDA-MB-231, NT2D1, human Rubicon-transfected 293T.

Predicted reactivity: Mouse (92%), Pig (94%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation. Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex. Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.