Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), NovaFluor Yellow 690, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), NovaFluor Yellow 690

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.2 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV / Violet / Yellow Series
• eFluor 450, 506, 660
• FITC
• PE, PE-Cyanine5, PE-Cyanine7
• Super Bright Series
• Request custom conjugation available

Product Specific Information

The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all developmental stages except plasma cells. Follicular dendritic cells also express CD19. Together with CD21, CD81, and MHC class II, CD19 forms a complex associated with the BCR, mediating tyrosine phosphorylation, calcium flux, and B cell proliferation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes (≤0.2 µg/test)

A test is defined as the amount of antibody staining a cell sample (100 µL volume, 10^5–10^8 cells/test). Antibody should be titrated for optimal performance.

Note:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01)

• Required for optimal staining with NovaFluor conjugates
• Add 5 µL per sample to antibody cocktails before combining with cells
• For single-color controls: 5 µL per 100 µL cell sample (10^3–10^8 cells)

NovaFluor conjugates use Phiton™ technology based on nucleic acid dye structures engineered for optimized spectral performance and minimized spillover.

Excitation: 552 nm
Emission: 690 nm
Laser: 561 nm (Yellow Laser)

Internal testing shows non-specific staining of B cells in SJL mice; not observed in BALB/c or C57BL/6 strains.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and some myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for the BCR, regulating B cell activation and proliferation. Mutations lead to hypogammaglobulinemia, while overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures.

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