Thermo Fisher Scientific CDC6 Monoclonal Antibody (OTI7B12), TrueMAB
CDC6 단백질을 인식하는 Mouse monoclonal 항체로, Western blot 및 IHC(P) 실험에 적합. 정제된 Lyophilized 형태로 제공되며, PBS buffer에 8% trehalose 함유. 세포주기 G1~S 전이 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7B12 |
| Immunogen | Full length human recombinant CDC6 protein produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The CDC6 protein is homologous to Saccharomyces cerevisiae Cdc6, essential for the initiation of DNA replication.
It acts as a regulator during early steps of DNA replication, localizing to the nucleus during G1 phase and translocating to the cytoplasm at the start of S phase.
This translocation is regulated by phosphorylation via Cdks, and CDC6 transcription is controlled by E2F proteins in response to mitogenic signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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