Thermo Fisher Scientific TEF1 Polyclonal Antibody
TEF1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, ChIP 등 다양한 응용에 사용 가능. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. 1 mg/mL 농도의 액상 형태로, 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View publication |
| Immunohistochemistry (IHC) | – | View publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | – |
| ChIP assay (ChIP) | 1:20–1:50 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 105–150 of human TEF-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553899 |
Product Specific Information
This antibody detects endogenous TEF1 protein at a molecular weight of approximately 48 kDa.
Purity is greater than 95% as determined by SDS-PAGE.
Target Information
TEF1 (Transcriptional Enhancer Factor 1) is a nuclear protein belonging to the TEA/ATTS domain family. It is expressed in numerous cell types and regulates the expression of various genes.
TEF-1 binds to GT-IIC, SphI/II, and M-CAT motifs, as well as the proximal regulatory element (PRE) of transforming growth factor-alpha, which is overexpressed in many cancers.
TEF-1 can repress transcription in placental cells and is phosphorylated by PKC isozymes both in vitro and in vivo, reducing its DNA-binding capability.
It also forms complexes with tumor antigen (TAg), suggesting a potential role in tumorigenesis. Dimerization of TEF-1 may be essential for its function as a transcriptional regulator.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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