Thermo Fisher Scientific Phospho-DENND3 (Thr450) Polyclonal Antibody
인산화된 Thr450 부위의 DENND3 단백질을 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot에 적합하며, 항원 친화 크로마토그래피로 정제됨. 인산화 신호전달 연구 및 오토파지 관련 단백질 분석에 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr450 conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen Affinity Chromatography |
| Storage Buffer | 10mM HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2807469 |
Product Specific Information
Antigen affinity purified from pooled serum.
Target Information
DENND3 is a guanine nucleotide exchange factor (GEF) activating RAB12. It promotes GDP-GTP exchange, converting inactive GDP-bound RAB12 into its active GTP-bound form (PubMed:20937701).
It regulates autophagy in response to starvation via RAB12 activation. Starvation triggers ULK1/2-dependent phosphorylation at Ser-472 and Ser-490, enabling recruitment of 14-3-3 adapter proteins and enhancing GEF activity toward RAB12.
DENND3 also mediates protein transport from recycling endosomes to lysosomes, influencing degradation of transferrin receptor and amino acid transporter PAT4 (PubMed:20937701).
Phosphorylation at Tyr-858 under starvation further upregulates GEF activity and initiates autophagy. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: PA5-95667_DENND3_A2RUS2-1_Rabbit.svg, PA5-95667_DENND3_A2RUS2-1_Rabbit_PDP.jpeg)
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