Thermo Fisher Scientific Phospho-Histone H3 (Ser10, Thr11) Polyclonal Antibody
인산화된 Histone H3 (Ser10, Thr11)을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB, IHC, ICC/IF, ChIP 등 다양한 응용 가능. 높은 특이성과 재현성 제공. 연구용으로만 사용.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [p Ser10, p Thr11] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phosphorylated peptide surrounding Serine 10 and Threonine 11 of human Histone H3.2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Histone H3 [p Ser10, p Thr11] reacts with human Histone H3.2. A BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans. Predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones alter chromatin structure or facilitate transcription factor binding. The N-terminal tail of histone H3 can undergo methylation, acetylation, or phosphorylation, influencing cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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