Thermo Fisher Scientific UBE2E3 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Product Specifications

Product Specific Information

• Recommended positive control: mouse muscle
• Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (93%), Xenopus laevis (98%), Chicken (99%), Rhesus Monkey (99%), Bovine (100%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.
The first step requires ATP-dependent activation of the Ub C-terminus and assembly of multi-Ub chains by the Ub-activating enzyme (E1). The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex.
The Ub-ligase (E3) catalyzes the transfer of Ub from E2 to the appropriate protein substrate.
UBE2E3 (also known as UBCH9) catalyzes the covalent attachment of ubiquitin to other proteins and is involved in the regulation of transepithelial sodium transport in renal cells. It may also play a role in cell growth arrest.
UBE2E3 shuttles between the cytoplasm and nucleus in an IPO11-dependent manner, is ubiquitously expressed at low levels, and is highly expressed in skeletal muscle.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.