Thermo Fisher Scientific YBX1 Recombinant Rabbit Monoclonal Antibody (9J7M4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000

-

Immunocytochemistry (ICC/IF)

1:200-1:800

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

9J7M4

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 225-324 of human YB-1/YBX1 (P67809). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:YBX1,uniProtId:P67809-1,ncbiNodeId:9606,antigenRange:225-324,antigenLength:324,antigenImageFileName:MA5-42819_YBX1_P67809-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42819_YBX1_P67809-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911960

Product Specific Information

Positive test controls include: Mouse testis, Rat heart, HeLa. The target is usually found in the following locations: Cytoplasm, Cytoplasmic granule, Nucleus, Secreted.

Immunogen sequence: GAGEQGRPVR QNMYRGYRPR FRRGPPRQRQ PREDGNEEDK ENQGDETQGQ QPPQRRYRRN FNYRRRRPEN PKPQDGKETK AADPPAENSS APEAEQGGAE

Target Information

YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. This protein binds and stabilizes cytoplasmic mRNA and contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. It binds to promoters that contain a Y-box (5-CTGATTGGCCAA-3), such as HLA class II genes. It regulates the transcription of numerous genes and promotes separation of DNA strands that contain mismatches or are modified by cisplatin. It has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro), and it may play a role in DNA repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.