Thermo Fisher Scientific YBX1 Recombinant Rabbit Monoclonal Antibody (9J7M4)
YBX1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 고순도 친화 크로마토그래피 정제 및 안정한 PBS 저장용액 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:800 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 9J7M4 |
| Immunogen | Synthetic peptide within amino acids 225–324 of human YB-1/YBX1 (UniProt ID: P67809) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS (pH 7.3), 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911960 |
Product Specific Information
- Positive test controls: Mouse testis, Rat heart, HeLa cells
- Subcellular localization: Cytoplasm, Cytoplasmic granule, Nucleus, Secreted
Immunogen sequence:
GAGEQGRPVR QNMYRGYRPR FRRGPPRQRQ PREDGNEEDK ENQGDETQGQ QPPQRRYRRN FNYRRRRPEN PKPQDGKETK AADPPAENSS APEAEQGGAE
Target Information
YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. It stabilizes cytoplasmic mRNA and modulates translation by influencing interactions with eukaryotic initiation factors. YBX1 binds promoters containing a Y-box sequence (5'-CTGATTGGCCAA-3'), such as HLA class II genes, and regulates transcription of numerous genes. It promotes DNA strand separation in mismatched or cisplatin-modified DNA, exhibits endonucleolytic activity, and may participate in DNA repair.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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