Thermo Fisher Scientific Prolactin Receptor Monoclonal Antibody (1A2B1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 21 publications 21 publications

Immunohistochemistry (IHC)

5-10 µg/mL

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 5 publications 5 publications

Flow Cytometry (Flow)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

5-10 µg/mL

View 3 publications 3 publications

Neutralization (Neu)

-

View 1 publication 1 publication

in situ PLA (PLA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

1A2B1

Immunogen

Recombinant human prolactin receptor protein containing the extracellular domain (ECD).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533231

Product Specific Information

This antibody is predicted to react with mouse and rat and reacts with the long form of the human prolactin receptor (hPRLr) protein at ~85-90 kDa, and may also identify the human prolactin receptor intermediate and delta S1 isoforms by western blotting. On immunoprecipitation, this antibody strongly recognizes only the long form of hPRLr. During development reactivity was confirmed with human T-47D breast carcinoma cells.

Target Information

Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.