Invitrogen™

Alkaline Phosphatase (ALP) Activity Kit

The Alkaline Phosphatase (ALP) Activity Kit is a highly reliable and sensitive assay designed for the quantitative measurement of ALP자세히 알아보기

The Alkaline Phosphatase (ALP) Activity Kit is a highly reliable and sensitive assay designed for the quantitative measurement of ALP activity in various sample types. ALP is a group of cytomembrane-related enzymes with hydrolysis and transfer activity, acting on a variety of phosphate substrates. ALP is a homologous dimerase where each catalytic site contains three metal ions.

The assay is based on the ability of alkaline phosphatase to decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol reacts with 4-aminopyrine in alkaline solution and oxidizes with potassium ferricyanide to form a red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

사양

어세이 범위0.13–50 King Units/100 mL

어세이 민감성0.13 King Units/100 mL

검출 방법Colorimetric

샘플 종류Plasma, Serum, Tissue Homogenates, Whole Cells

용도(장비)Microplate Reader

인큐베이션 시간15 min.

분석 간 CV8.5

분석 내 CV5.1

수량96 tests

샘플 부피5 μL

배송 조건Dry Ice, 2–8°C

타겟 키트 이름 지정Alkaline Phosphatase (ALP)

총 어세이 시간30 min.

Unit SizeEach