Thermo Fisher Scientific KIF3A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 430-699 of human KIF3A (NP_008985.3). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KIF3A,uniProtId:Q9Y496-1,ncbiNodeId:9606,antigenRange:430-699,antigenLength:699,antigenImageFileName:PA5-121019_KIF3A_Q9Y496-1_Rabbit.svg,antigenImageFileNamePDP:PA5-121019_KIF3A_Q9Y496-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.38 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914591

Product Specific Information

Positive test controls include: MCF-7, SW480, 293T, SKOV3, HeLa, Mouse brain, Mouse ovary, Rat liver, Rat brain. The target is usually found in the following locations: Cell projection, Cytoplasm, cilium, cytoskeleton.

Immunogen sequence: RKALETKLDM EEEERNKARA ELEKREKDLL KAQQEHQSLL EKLSALEKKV IVGGVDLLAK AEEQEKLLEE SNMELEERRK RAEQLRRELE EKEQERLDIE EKYTSLQEEA QGKTKKLKKV WTMLMAAKSE MADLQQEHQR EIEGLLENIR QLSRELRLQM LIIDNFIPRD YQEMIENYVH WNEDIGEWQL KCVAYTGNNM RKQTPVPDKK EKDPFEVDLS HVYLAYTEES LRQSLMKLER PRTSKGKARP KTGRRKRSAK PETVIDSLLQ

Target Information

The kinesins constitute a large family of microtubule-dependent motor proteins which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell. Individual kinesin members play crucial roles in cell division, intracellular transport and membrane trafficking events including endocytosis and transcytosis. Members of the heterotrimeric kinesin II family of microtubule associated motors generally contain two different motor subunits from the KIF3 family, which includes KIF3A, B and C. KIF3 isoforms mediate anterograde transport of membrane bound organelles in neurons and melanosomes, transport between the endoplasmic reticulum and the Golgi, and transport of protein complexes within cilia and flagella required for their morphogenesis. KIF3A may influence neurogenesis at the level of embryonic cellular events, where the asymmetry of the genetic control circuit controlling left-right (L-R) axis determination is defined. Loss of KIF3A function in mice photoreceptors causes apoptotic cell death, suggesting that kinesin II mediated transport is required for proper cell fate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.