Thermo Fisher Scientific SF3A1 Polyclonal Antibody
SF3A1 단백질을 표적으로 하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 고순도 항원 친화 크로마토그래피 정제. 인간, 마우스, 랫트 반응성. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human SF3A1. Recombinant protein control fragment (Product #RP-91384). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2647173 |
Product Specific Information
Immunogen sequence:
TEDSLMPEEE FLRRNKGPVS IKVQVPNMQD KTEWKLNGQV LVFTLPLTDQ VSVIKVKIHE ATGMPAGKQK LQYEGIFIKD SNSLAYYNMA NGAVIHLALK ESGGRKK
Highest antigen sequence identity to the following orthologs:
- Mouse: 97%
- Rat: 97%
Target Information
This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2, and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family, named for the SURP motifs that mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half, while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies show the two SURP motifs are necessary for binding to subunit 3, while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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