Thermo Fisher Scientific Acid Phosphatase Polyclonal Antibody
Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합한 Acid Phosphatase 폴리클로날 항체. Human, Mouse, Rat 반응성. Rabbit IgG 기반으로 Protein A 정제, 고순도 액상 형태. -20°C 보관, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human Acid Phosphatase (amino acids 71–158) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Low molecular weight protein tyrosine phosphatases, including the human red cell phosphatase HCPTP, are widely expressed cytosolic proteins (~18 kDa) that exist in distinct isoforms and are highly selective for phosphotyrosine over phosphoserine. The HCPTP-A and HCPTP-B isoforms expressed in human red cells and placenta correspond to fast and slow forms of red cell acid phosphatase. Like other tyrosine phosphatases, HCPTP is inhibited by vanadate and has a catalytic mechanism involving a cystinylphosphate intermediate. The bovine heart enzyme BHPTP structure shows a four-stranded central parallel β-sheet with flanking α-helices and an active site cysteine residue in the typical tyrosine phosphatase motif (CXXXXXR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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