Thermo Fisher Scientific GeneChip Human Gene 1.1 ST Array Plate, 1 x 96 array plate

Applied Biosystems™ GeneChip™ Human Gene 1.1 ST Array Plate

The Human Gene 1.1 ST 24-Array Plate and Trays provide the most accurate, sensitive, and comprehensive measurement of protein-coding and long intergenic non-coding RNA (lincRNA) transcripts.

Comprehensive Design

Whole-transcript arrays include probes to measure both messenger RNA (mRNA) and long intergenic non-coding RNA transcripts (lincRNA). These designs enable a complete expression profile of mRNA as well as intermediary lincRNA transcripts that influence mRNA expression.

Over the past 20 years, research has focused on protein-coding mRNA transcripts and their role in cellular processes such as disease and development. Recently, more than 10,000 transcripts (>200 bases) with little or no protein-coding potential have been identified. Although only a small fraction of these non-coding RNAs have functional annotations, evidence shows that differential expression of lincRNAs plays a key role in disease genesis and progression, including cancer.

Recent transcriptome profiling advancements have revealed associations of lincRNAs in diverse cellular functions such as:

• Regulation of mRNA transcription
• Regulation of mRNA post-transcriptional modifications
• Occlusion or recruitment of transcription factor binding
• Activation and transportation of transcription factors
• Interaction with accessory proteins
• Guiding protein complexes to genomic locations

Key Benefits

• Comprehensive coverage for discovery of novel biological insights

  • 30,000 coding transcripts

  • 11,000 long intergenic non-coding transcripts
• Detect alternative splicing events and transcript variants with optimized exon coverage
• High reproducibility: Intra-lot correlation coefficient = 0.99

Content Profile

The Human Gene 1.1 ST Array Plates were designed to measure differential expression of both coding and non-coding RNA transcripts. As new lincRNAs have been identified, the Human Gene 2.1 ST Array Plates (sold separately) were developed to extend coverage.
To supplement RefSeq lincRNA data, sequences and transcripts from lncRNA db (www.lncrnadb.com) and Broad Institute Human Body Map lincRNAs and TUCP catalog (http://www.broadinstitute.org/genome_bio/human_lincrnas/) were incorporated.

제품 사양