Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), NovaFluor Red 700, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), NovaFluor Red 700, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.1 µg)/test
Publications: View 1 publication

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV, Violet, Yellow Series
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright Series
• Request custom conjugation

Product Specific Information

The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all stages of development except plasma cells. Follicular dendritic cells also express CD19. CD19 forms a multimolecular complex with CD21, CD81, Leu13, and MHC class II, associating with BCR to induce tyrosine phosphorylation, calcium flux, and B cell proliferation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry of normal human peripheral blood cells, 5 µL (0.1 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for best results.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL per sample to antibody cocktails before combining with cells.

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures minimizing spillover and spread impacts.
Excitation: 639 nm
Emission: 700 nm
Laser: 633–640 nm (Red)

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and some myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for the B cell receptor (BCR), regulating normal and malignant B cell proliferation. Mutations can cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures.

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