Thermo Fisher Scientific FOXP3 Monoclonal Antibody (NRRF-30), eBioscience
마우스 FOXP3 단백질을 인식하는 NRRF-30 단클론 항체로, Treg 세포 연구에 적합. Western blot, IHC(F), Flow cytometry 등 다양한 응용 가능. 고순도(>90%) 및 0.2 µm 여과 처리. PBS(pH 7.2) 용액, 4°C 보관.
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3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific FOXP3 Monoclonal Antibody (NRRF-30), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 10 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | - | View 19 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NRRF-30 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865081 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats:
Product Specific Information
The NRRF-30 antibody reacts with mouse Foxp3 (FORKHEAD BOX P3, SCURFIN, JM2). Cross-reactivity with other proteins has not been determined. Foxp3 is a 49–55 kDa transcriptional regulator, identified as the gene defective in scurfy (sf) mice. High expression of Foxp3 mRNA is found in CD4+/CD25+ regulatory T cells (Treg), and ectopic expression in CD4+/CD25− cells induces a Treg phenotype.
Immunoblotting with NRRF-30 maps the epitope to amino acids 1–75 of mouse Foxp3. Intracellular staining of mouse splenocytes using fluorochrome-conjugated NRRF-30 with eBioscience Foxp3 Staining Buffers reveals approximately 3% of total cells in C57Bl/6 and 5% in BALB/c strains. Multicolor flow cytometry shows ~90% of CD4+/CD25+ cells and 4% of CD4+/CD25− cells stained with NRRF-30. Co-staining with FJK-16s (anti-mouse/rat Foxp3, Product #71-5775-40) shows 100% correlation.
Applications Reported:
- Immunoblotting (WB)
- Immunohistology of frozen tissue sections
Applications Tested:
- WB at 2 µg/mL for mouse splenocyte lysates
- IHC(F) ≤10 µg/mL
Titrate antibody for optimal performance in specific assays.
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is 381 amino acids long and highly expressed in CD25+CD4+ regulatory T cells, weakly in CD4+CD25− cells, and absent in CD4−CD8+ T cells. FOXP3 acts as a master regulatory gene and marker of regulatory T cells. Defects cause the scurfy phenotype in mice and IPEX syndrome in humans (immune dysfunction, polyendocrinopathy, enteropathy, X-linked). FOXP3 expression in human cancer cells is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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