Thermo Fisher Scientific CD180 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Despite its predicted molecular weight, RP105 often migrates at 95–105 kDa in SDS-PAGE.
A suggested positive control is human spleen tissue lysate.
PA5-20250 can be used with blocking peptide PEP-0372.

Target Information

CD180 (also known as RP105, Bgp95, LY64) is a type I membrane glycoprotein belonging to the Toll-like receptor (TLR) family.
Its cytoplasmic tail is short and lacks the TIR domain found in other TLRs. CD180 expression depends on coexpression with its helper molecule MD-1 and parallels that of TLR4 on antigen-presenting cells.
It regulates recognition of LPS and signaling in B cells by interacting with the TLR4 signaling complex, inhibiting microbial ligand binding.
Ligation of CD180 by monoclonal antibodies triggers B cell activation, upregulation of CD80/CD86, and increases cell size.
CD180 consists of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail, forming part of the RP105/MD-1 receptor complex.
Dysfunction of CD180 has been associated with Kawasaki Disease in children and may contribute to rheumatic disease pathology.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.