Thermo Fisher Scientific SPT3 Polyclonal Antibody
Thermo Fisher Scientific의 SPT3 폴리클로날 항체는 인간 SPT3H 단백질의 N-말단 펩타이드(aa 87-136)를 면역원으로 제작되었습니다. Western blot과 IHC(P) 실험에 적합하며, Protein A로 정제된 액상 형태입니다. PBS 버퍼와 sucrose 포함, -20°C 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 4–8 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide directed towards the N-terminal of human SUPT3H (aa 87–136) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 2% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2610047 |
Product Specific Information
Peptide sequence:
MRKDKKKLRR LLKYMFIRDY KSKIVKGIDE DDLLEDKLSG SNNANKRQKI
Sequence homology:
Cow: 100%
Dog: 100%
Goat: 100%
Guinea Pig: 86%
Horse: 100%
Human: 100%
Mouse: 93%
Pig: 100%
Rabbit: 86%
Rat: 93%
Target Information
The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from delta sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter, allowing TBP to bind to SAGA-dependent promoters.
Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3, and TRP3, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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