Thermo Fisher Scientific CD163 Monoclonal Antibody (M130, 2163)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, belonging to the scavenger receptor cysteine-rich superfamily. It acts as a receptor for the hemoglobin-haptoglobin complex, protecting tissues from oxidative damage and contributing to iron recycling through endocytosis of hemoglobin/haptoglobin and heme breakdown.

CD163 is expressed on human monocytes and macrophages, primarily during the late phase of inflammation. It is found on all circulating monocytes and most tissue macrophages except in mantle zones, germinal centers, interdigitating reticulum cells, and Langerhans cells. CD163 expression correlates with CD14 and CD64 positivity and is upregulated by IL-10, IL-6, and dexamethasone. LPS and PMA induce shedding of CD163 into plasma or cell supernatant.

CD163 binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner, showing higher affinity for HP1F phenotype complexes. It also triggers intracellular signaling cascades involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL-6 and CSF1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.