Thermo Fisher Scientific NFS1 Polyclonal Antibody
Human NFS1 단백질을 인식하는 Rabbit Polyclonal Antibody로, IHC(P) 및 ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 형태이며, 0.2 mg/mL 농도. 심장 및 골격근 등에서 높은 발현을 보이는 NFS1 연구용에 적합.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Immunocytochemistry (ICC/IF)
- Tested Dilution: 0.25–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NFS1. Recombinant protein control fragment (Product #RP-103109). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2644650 |
Product Specific Information
Immunogen sequence:
GVGAIYIRRR PRVRVEALQS GGGQERGMRS GTVPTPLVVG LGAACEVAQQ EMEYDHKRIS KLSERLIQNI MKSLPDVVMN GDPKHHYPGC INLSF
Highest antigen sequence identity to the following orthologs:
- Mouse: 95%
- Rat: 95%
Target Information
NFS1 (nitrogen fixation 1), also known as NIFS or IscS (cysteine desulfurase), is a member of the class V pyridoxal-phosphate-dependent aminotransferase family.
It localizes to the cytoplasm or mitochondrion depending on cytosolic pH.
Highest expression levels are found in heart and skeletal muscle, with lower levels in liver, brain, and pancreas.
NFS1 catalyzes the removal of sulfur from cysteine to form alanine, supplying inorganic sulfur for iron-sulfur (Fe-S) clusters.
Fe-S clusters act as essential cofactors in electron transfer during oxidative phosphorylation, enzymatic catalysis (e.g., aconitase, dehydratases), and DNA repair (endonuclease III).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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