Thermo Fisher Scientific Sin1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
• Predicted to react with Mouse, Rat, Chicken, Sheep, Bovine, and Orangutan based on 100% sequence identity

Target Information

Sin1 was initially identified as the human homolog of S. pombe SIN1. It is identical to Mip1, a protein that interacts with MEKK2, a member of the MAPK intracellular signaling network. MAPKAP1 prevents MEKK2 activation and dimerization by forming a complex with inactive, non-phosphorylated MEKK2, thereby blocking JNK1/2, ERK1/2, p38, and ERK5 MAPKs.

MAPKAP1 also plays a role in the TOR signaling pathway, which regulates cell growth and proliferation in response to nutrients, growth factors, and hormones. It helps maintain the TOR/rictor assembly but not the TOR/RAPTOR complex, enabling specific phosphorylation of Akt, linking growth factor–PI3K signaling to nutrient-regulated TOR signaling. Multiple alternatively spliced isoforms of MAPKAP1 have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.