Thermo Fisher Scientific ATF2 Polyclonal Antibody
ATF2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 적합합니다. Human 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS/glycerol buffer에 보관되며 연구용으로 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ATF2 (Product #RP-100704) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789948 |
Product Specific Information
Immunogen sequence:
SSNVHVPAAV PLVRPVTMVP SVPGIPGPSS PQPVQSEAKM RLKAALTQQH PPVTNGDTVK GHGSGLVRTQ SEESRPQSLQ QPATSTTETP ASPAHTTPQT QSTSGRRRRA ANEDPDEKRR KFLERNRAAA SRCRQKRKVW VQS
Target Information
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and belongs to the ATF/CREB family of leucine zipper proteins.
ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways.
Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2.
Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in transfected cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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