Thermo Fisher Scientific STIM1 (extracellular) Polyclonal Antibody, Atto 550

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:20

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:20

Product Specifications

Product Specific Information

• Reconstitution: Add 50 µL double distilled water (DDW), depending on sample size.
• Ships as lyophilized powder at room temperature.
• Upon arrival, store at -20°C.
• Reconstituted solution can be stored at 4°C for up to 1 week.
• For longer storage, aliquot and keep at -20°C.
• Avoid repeated freeze/thaw cycles.
• Centrifuge all antibody preparations before use (10,000 × g, 5 min).

Target Information

STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. STIM1 also associates with growing ends of microtubules and contributes to endoplasmic reticulum tubule extension.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.