Thermo Fisher Scientific STIM1 (extracellular) Polyclonal Antibody, Atto 550
인간, 마우스, 랫트에서 반응하는 STIM1 폴리클로날 항체로 Atto 550 형광 표지. 면역조직화학 및 면역세포화학 분석에 적합. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 신뢰성 제공. 연구용으로만 사용 가능.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:20
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:20
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHSEDEKLSFEAVR, corresponding to amino acid residues 56–69 of human STIM1 (Extracellular, N-terminus) |
| Conjugate | Atto™ 550 |
| Excitation / Emission Max | 554 / 575 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution: Add 50 µL double distilled water (DDW), depending on sample size.
- Ships as lyophilized powder at room temperature.
- Upon arrival, store at -20°C.
- Reconstituted solution can be stored at 4°C for up to 1 week.
- For longer storage, aliquot and keep at -20°C.
- Avoid repeated freeze/thaw cycles.
- Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. STIM1 also associates with growing ends of microtubules and contributes to endoplasmic reticulum tubule extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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